https://video.igem.org/videos/watch/34bd3612-497c-4372-ae62-fcfd84136d93 Title: STING: Small TnpB-based Innovative Nuclease toolkit for bacterial Genome editing Description: STING develops a bacterial genome-engineering toolkit based on the miniature ISHahl1 TnpB nuclease. The system achieved gene deletion in E. coli (4.2–100%) and P. putida (30–44%), alongside transcriptional repression and cytosine base editing. Structure-guided evolution expanded recognition to five noncanonical TAMs. Computational modelling integrated Protenix, Gaussian-mixture scoring, and molecular dynamics simulations to optimize TAM recognition. Integrated Human Practices incorporated feedbacks from experts and stakeholders to improve functionality and biosafety. A self-targeting biocontainment system reduced bacterial survival by up to five orders of magnitude. Core parts include ISHahl1 TnpB (BBa_26F4MI62) and variants with expanded TAM specificities, programmable reRNA, inactive TnpB variants, and base-editing modules, establishing a versatile miniature genome-engineering platform. Wiki | Official Page Thu, 29 Oct 2026 22:26:57 GMT https://validator.w3.org/feed/docs/rss2.html PeerTube - https://video.igem.org https://video.igem.org/client/assets/images/icons/icon-1500x1500.png https://video.igem.org/videos/watch/34bd3612-497c-4372-ae62-fcfd84136d93 All rights reserved, unless otherwise specified in the terms specified at https://video.igem.org/about and potential licenses granted by each content's rightholder.