https://video.igem.org/videos/watch/36cfd8e3-1436-4534-b163-41f0cfc1a4ac Breaking the operon does not tear the pili off. It stops them being replaced. Without srtC1 there is no sortase to pin new subunits to the cell wall, so existing pili are shed and never rebuilt. The cell loses its grip on the mucus layer and goes with the flow, with its DEHP load still inside it. Release follows from one cut. No second actuator was engineered. 00:00 No new pilus subunits 00:07 Adhesion lost, and the cell lets go Chapter 6 of 7 from the FSU iGEM 2026 project Lilieum. https://2026.igem.wiki/fsu Tue, 20 Oct 2026 02:55:17 GMT https://validator.w3.org/feed/docs/rss2.html PeerTube - https://video.igem.org https://video.igem.org/client/assets/images/icons/icon-1500x1500.png https://video.igem.org/videos/watch/36cfd8e3-1436-4534-b163-41f0cfc1a4ac All rights reserved, unless otherwise specified in the terms specified at https://video.igem.org/about and potential licenses granted by each content's rightholder.