https://video.igem.org/videos/watch/466b3f59-cd3f-4135-b95b-b3f7fab68aa9 Title: FlaminGone: An Engineered Amyloid Coating for Sustainable Fire Retardancy Description: Traditional chemical fire retardants pose severe health and environmental hazards, creating an urgent need for eco-friendly alternatives. FlaminGone delivers a sustainable bio-coating platform using E. coli’s CsgA curli subunit as a self-assembling amyloid scaffold. Fusing hydrophobic BslA for weatherproofing and polyethylene-binding peptides (PEbp) for substrate anchoring, our Two-Layer System (CsgA-PEbp + CsgA-BslA + APP/EG) overcomes secretion limits and steric hindrance. To accelerate construct engineering, we developed PyroForge, a sequence-only machine learning platform using ESM-2 features to predict protein melting temperature, folding free energy, and mutational stability. Under fire testing, the bio-matrix forms a dense char shield that halts flame propagation and self-extinguishes. BslA prevents rain washout across weathering, while Arabidopsis assays confirm zero ecotoxicity. Swapping binding domains extends this platform to diverse industrial materials. Wiki | Official Page Thu, 29 Oct 2026 22:21:35 GMT https://validator.w3.org/feed/docs/rss2.html PeerTube - https://video.igem.org https://video.igem.org/client/assets/images/icons/icon-1500x1500.png https://video.igem.org/videos/watch/466b3f59-cd3f-4135-b95b-b3f7fab68aa9 All rights reserved, unless otherwise specified in the terms specified at https://video.igem.org/about and potential licenses granted by each content's rightholder.