https://video.igem.org/videos/watch/554bb4f4-607f-4463-af75-e8d3f6440878 Title: Smart Hydrogel for Integrated Enamel & Lesion Defence Description: Orthodontic appliances improve alignment and aesthetics but impede oral cleaning. Patients clean 3–4 times daily, often for >15 min, and compliance is poor; >70% report cleaning difficulty, only ~20% maintain good oral hygiene, and >60% develop plaque or gingival problems. Cariogenic acid causes enamel demineralization, leading to white spot lesions (WSLs), caries, and gingivitis; WSL prevalence in orthodontic patients reaches 55.06%. Existing regimens provide only transient protection and lack combined antibacterial and remineralization functions; 83.14% of surveyed respondents would try a smart hydrogel coating. We developed SHIELD, an acid-responsive antimicrobial peptide hydrogel coating for braces. CBM106 anchors ALP in alginate hydrogel, catalyzing CaGP hydrolysis to deposit nHA in situ and form a mineralized layer. This layer and the hydrogel network create a dual barrier for sustained drug release and calcium-mineral competition against acid attack. An N-terminal fusion tag, L6KD-PT-PEP-MtuI, stably encapsulates AMP as active inclusion bodies; pH < 6.5 triggers MtuI self-cleavage and slow AMP release for long-acting antibacterial activity. PhoK and LL-37 were screened, and fusion protein construction, acid-responsive release, and dual-barrier sustained release were validated. SHIELD aims to block irreversible demineralization, reduce complications and costs, and provide long-acting, safe, and convenient protection, supporting the WHO oral health strategy and the SDGs. Wiki | Official Page Thu, 29 Oct 2026 21:21:48 GMT https://validator.w3.org/feed/docs/rss2.html PeerTube - https://video.igem.org https://video.igem.org/client/assets/images/icons/icon-1500x1500.png https://video.igem.org/videos/watch/554bb4f4-607f-4463-af75-e8d3f6440878 All rights reserved, unless otherwise specified in the terms specified at https://video.igem.org/about and potential licenses granted by each content's rightholder.