https://video.igem.org/videos/watch/5ff5e8cb-80c5-4f3c-b1a8-9782d3b00f5c While nuclear genome engineering has advanced rapidly, mitochondrial gene editing remains fundamentally unexplored, current methods being limited to specific single nucleotide substitutions. Prime editing is a gene editing system consisting of a Cas nickase fused to a reverse transcriptase (RT) and a prime editing guide RNA (pegRNA) used as a template to synthesize a new, edited strand without introducing double-strand DNA breaks. Our project Primetheus adapts this system for mitochondria through separate import of the fused Cas-RT complex and the pegRNA into mitochondria. To overcome the technical challenge of verifying intra-mitochondrial RNA delivery, we are also developing a novel, genetically encoded RNA-based readout system to enable continuous live-cell RNA monitoring. Primetheus will enable researchers to introduce a wider range of edits, including all base substitutions, as well as longer insertions into mitochondrial DNA, allowing in-depth studies of the mitochondrial genome and unlocking new therapeutic approaches. https://2026.igem.wiki/munich Mon, 19 Oct 2026 02:50:02 GMT https://validator.w3.org/feed/docs/rss2.html PeerTube - https://video.igem.org https://video.igem.org/client/assets/images/icons/icon-1500x1500.png https://video.igem.org/videos/watch/5ff5e8cb-80c5-4f3c-b1a8-9782d3b00f5c All rights reserved, unless otherwise specified in the terms specified at https://video.igem.org/about and potential licenses granted by each content's rightholder.