https://video.igem.org/videos/watch/7f347145-b607-4283-b77b-41b454dba44a Title: PanCrisp Description: Pancreatic ductal adenocarcinoma is the sixth leading cause of cancer death worldwide and the third in the United States. Caught early, most patients are alive five years later. Across all stages, very few are. The difference is not the treatment — it is the timing. And unlike breast or colon cancer, there is no routine test looking for it. PanCrisp is a CRISPR-Cas13a saliva assay designed to detect pancreatic cancer early, using a five-microRNA panel with one guide per tube. This video covers: • Why PDAC is found too late, and what that costs • How conversations with clinicians, scientists and regulators shaped every core design decision • The Cas13a assay, our guide and reporter design, and the controls behind it • Two engineering cycles — including the ordering error that gave us no signal, and how we found it • Five computational models: genome mining, a Bayesian diagnostic network, ODE binding kinetics, limit of detection, and phone-camera detectability • Two open software tools, SpacerSentry and CasPlate Analyzer • Our cartridge and reader design, and the staged path from hospital labs toward routine screening Video attribution: Music: Generated with Google Flow. No third-party copyrighted recordings were used. Tools: Claude (script editing, animation), Google Flow (AI video generation), CapCut (editing ) Music: From the CapCut built-in audio library, used under CapCut’s licence terms. Voices: Trishona, Anagha, Leo, Sattwik Dutta, Aarthi Wiki | Official Page Thu, 29 Oct 2026 22:24:33 GMT https://validator.w3.org/feed/docs/rss2.html PeerTube - https://video.igem.org https://video.igem.org/client/assets/images/icons/icon-1500x1500.png https://video.igem.org/videos/watch/7f347145-b607-4283-b77b-41b454dba44a All rights reserved, unless otherwise specified in the terms specified at https://video.igem.org/about and potential licenses granted by each content's rightholder.