https://video.igem.org/videos/watch/800f99f0-8de3-40f2-95e9-0ea7eec28162 Title: Dual EcN Probiotic Synergistic Therapy for the Rare Disease Hyperlysinemia Description: Hyperlysinemia is a rare amino acid metabolic disorder. Patients cannot break down lysine in their bodies and must maintain a lifelong low-protein diet. This condition causes severe complications including brain developmental delay and epilepsy, trapping patients in a lifelong “dietary prison”. Our project presents dual engineered EcN probiotics for synergistic therapy. We build lysine degradation pathways inside EcN strains to consume excess lysine. By applying the SpyTag/SpyCatcher system, we physically couple these two EcN strains to construct metabolic tunnels, mitigating low reaction efficiency and secondary toxicity from intermediate diffusion. A kill-switch is also integrated into our engineered strains to manage biosafety risks from bacterial leakage. Our therapy aims to free hyperlysinemia patients from strict lifelong dietary restrictions and deliver a promising new treatment option. Wiki | Official Page Thu, 29 Oct 2026 21:22:20 GMT https://validator.w3.org/feed/docs/rss2.html PeerTube - https://video.igem.org https://video.igem.org/client/assets/images/icons/icon-1500x1500.png https://video.igem.org/videos/watch/800f99f0-8de3-40f2-95e9-0ea7eec28162 All rights reserved, unless otherwise specified in the terms specified at https://video.igem.org/about and potential licenses granted by each content's rightholder.