https://video.igem.org/videos/watch/88a2d222-4860-4a2d-8418-70d9b10992f4 Title: DigiCAT：A Multi-Threshold Biosensor for Monitoring of Feline Diabetic Ketoacidosis Description: Converting continuous molecular signals into programmable genetic decisions remains a challenge in synthetic biology. We developed a modular multi-threshold biosensing platform using β-hydroxybutyrate (BHB), a biomarker associated with feline diabetic ketoacidosis, as a proof of concept. BDH converts BHB into acetoacetate (AcAc), which is sensed by the AtoSC two-component system to regulate the expression of Bxb1 and phiC31 integrases. By tuning integrase expression through ribosome-binding sites (RBSs) and integrating mathematical modeling to guide threshold design, our system aims to convert continuous molecular inputs into discrete genetic states and fluorescent outputs. This work provides a modular framework for programmable multi-threshold biosensing. Wiki | Official Page Thu, 29 Oct 2026 22:24:50 GMT https://validator.w3.org/feed/docs/rss2.html PeerTube - https://video.igem.org https://video.igem.org/client/assets/images/icons/icon-1500x1500.png https://video.igem.org/videos/watch/88a2d222-4860-4a2d-8418-70d9b10992f4 All rights reserved, unless otherwise specified in the terms specified at https://video.igem.org/about and potential licenses granted by each content's rightholder.