https://video.igem.org/videos/watch/89f3cffc-098b-45b8-b3cb-0cca14788cd6 Title: preCISe: a modular tool for intracellular delivery using contractile injection systems Description: Protein therapeutics offer unique advantages in terms of versatility and specificity, making them promising candidates for treating a wide range of diseases. However, their inability to efficiently cross cell membranes generally limits their access to intracellular targets. preCISe addresses this challenge with a platform for target-specific protein delivery based on extracellular contractile injection systems (eCISs), bacterial nanosyringes that inject cargo directly into the cytosol. The platform comprises a modular eCIS design that can be retargeted to new receptors by exchanging their binding domains, preCISion reporter cell lines that quantify intracellular delivery, and our deCISion software which finds cell-specific receptors and binders and returns ready-to-order DNA sequences. Because receptors unique to one cell type are rare, we initiated a dual-targeting strategy: the cargo is split between two eCISs addressing two receptors and reassembles only in cells displaying both. Our software OpenSplitProtein ranks split sites that preserve folding and spontaneous reassembly. Wiki | Official Page Thu, 29 Oct 2026 22:23:44 GMT https://validator.w3.org/feed/docs/rss2.html PeerTube - https://video.igem.org https://video.igem.org/client/assets/images/icons/icon-1500x1500.png https://video.igem.org/videos/watch/89f3cffc-098b-45b8-b3cb-0cca14788cd6 All rights reserved, unless otherwise specified in the terms specified at https://video.igem.org/about and potential licenses granted by each content's rightholder.