https://video.igem.org/videos/watch/94450b33-ce8e-48f2-a113-7ffc4ebcf9fa Diana is the free, open-source web app from iGEM Team UMA-Malaga 2026 for designing CRISPR-Cas13 guide RNAs. This tour starts from a disease and ends with a plate map: find its ClinVar variants (disease names and codes from the Monarch Initiative), design ranked spacers on both strands, check off-targets (Bowtie) and RNA folding (ViennaRNA), and build the complete gRNA with its FORNA diagram. It then shows the shortlist and exports, combinatorial pooling onto 96/384-well plates, and decoding a screen. Guides are computational candidates to be validated in the lab. Variant lists come from live ClinVar data and are not clinical statements. Source code (MIT license): https://gitlab.igem.org/2026/software/uma-malaga/diana-grna-designer https://2026.igem.wiki/uma-malaga/ Mon, 19 Oct 2026 02:53:43 GMT https://validator.w3.org/feed/docs/rss2.html PeerTube - https://video.igem.org https://video.igem.org/client/assets/images/icons/icon-1500x1500.png https://video.igem.org/videos/watch/94450b33-ce8e-48f2-a113-7ffc4ebcf9fa All rights reserved, unless otherwise specified in the terms specified at https://video.igem.org/about and potential licenses granted by each content's rightholder.