https://video.igem.org/videos/watch/9e9abd47-9a62-4a0a-a743-b8b60e9aae50 Title: SensePFASt Description: PFAS flow through our rivers and accumulate in our bodies. Europe has answered with strict limits. But the methods that enforce them are slow, expensive, and locked inside specialised laboratories. With SensePFASt, we took four parallel routes to a PFAS biosensor, each built for a different strength: sensitivity, speed, simplicity, and degradation. Our flagship sensor is six times more sensitive than the one it builds on. Alongside it, we designed luminescent hLFABP linkers in silico, tested dual stress promoters in E. coli, and built a yeast laccase platform for future degradation work. None of it happened in isolation. At every step we asked relevant stakeholders, from policymakers and water utilities to researchers and the public. Ultimately, we hope this idea keeps growing with the iGEM generations that follow us. Citations: [1] J. Beltrán et al., "Rapid biosensor development using plant hormone receptors as reprogrammable scaffolds," Nature Biotechnology, vol. 40, no. 12, Dec. 2022. doi: 10.1038/s41587-022-01364-5; [2] H. Tian et al., "Unusually broad-spectrum small-molecule sensing using a single protein scaffold," Proceedings of the National Academy of Sciences of the United States of America, vol. 122, no. 51, art. no. e2519924122, Dec. 2025. doi: 10.1073/pnas.2519924122; [3] M. M. Mann and B. W. Berger, "A genetically-encoded biosensor for direct detection of perfluorooctanoic acid," Scientific Reports, vol. 13, no. 1, art. no. 15186, Sep. 2023. doi: 10.1038/s41598-023-41953-1; [4] M. E. Wintenberg, O. B. Vasilyeva, and S. W. Schaffter, "Comparative transcriptomic analysis of perfluoroalkyl substances-induced responses of exponential and stationary phase Escherichia coli," BMC Genomics, Nov. 2025. doi: 10.1186/s12864-025-12109-4; [5] M. Hu and C. Scott, "Toward the development of a molecular toolkit for the microbial remediation of per- and polyfluoroalkyl substances," Applied and Environmental Microbiology, vol. 90, no. 4, art. no. e00157-24, Apr. 2024. doi: 10.1128/aem.00157-24 Wiki | Official Page Thu, 29 Oct 2026 22:24:32 GMT https://validator.w3.org/feed/docs/rss2.html PeerTube - https://video.igem.org https://video.igem.org/client/assets/images/icons/icon-1500x1500.png https://video.igem.org/videos/watch/9e9abd47-9a62-4a0a-a743-b8b60e9aae50 All rights reserved, unless otherwise specified in the terms specified at https://video.igem.org/about and potential licenses granted by each content's rightholder.