https://video.igem.org/videos/watch/a007b120-c344-4304-a75b-4e1d44632f9f Title: NariViva- Engineered Probiotics Delivering Naringenin to Restore Vaginal Health Description: Genitourinary Syndrome of Menopause (GSM) is a chronic condition associated with declining estrogen levels, affecting quality of life in postmenopausal women. NariViva aims to develop a probiotic-based biomedical platform for localized, pH-responsive naringenin production as a potential strategy for GSM management. We designed a genetic circuit integrating a pH-responsive regulatory module with a naringenin biosynthetic pathway comprising tyrosine ammonia-lyase (TAL), 4-coumarate-CoA ligase (4CL), chalcone synthase (CHS), and chalcone isomerase (CHI). Using Escherichia coli as an initial chassis, we characterized pH-responsive gene expression and investigated a naringenin-responsive regulatory module. Western blot and HPLC analysis showed increased CHI abundance with rising pH in the circuit without negative feedback, whereas the negative-feedback design helped stabilize CHI abundance across the tested pH range. These findings support the feasibility of combining environmental sensing with feedback regulation. Further product-level validation, chassis optimization, and biosafety evaluation are needed before future development in vaginally compatible probiotics. Wiki | Official Page Thu, 29 Oct 2026 22:23:40 GMT https://validator.w3.org/feed/docs/rss2.html PeerTube - https://video.igem.org https://video.igem.org/client/assets/images/icons/icon-1500x1500.png https://video.igem.org/videos/watch/a007b120-c344-4304-a75b-4e1d44632f9f All rights reserved, unless otherwise specified in the terms specified at https://video.igem.org/about and potential licenses granted by each content's rightholder.