https://video.igem.org/videos/watch/a30c979a-6ad9-445a-8eb4-642071920339 Title: CirCure: A Multi-Epitope Circular RNA Vaccine Platform for Triple-Negative Breast Cancer Description: Triple-negative breast cancer (TNBC) is hard to treat: its tumors are heterogeneous, recur often, and escape single-target therapies. CirCure is a modular circular RNA (circRNA) vaccine platform that broadens immune recognition beyond a single TNBC-associated antigen. A computational pipeline screens antigen fragments, or epitopes, for MHC-I binding, immunogenicity, and structural compatibility, prioritizing candidates with strong potential to activate CD8+ "killer" T cells. The selected epitopes are encoded into a single engineered circRNA. Inside dendritic cells, the circRNA acts as a durable instruction set, supporting antigen production and presentation so CD8+ T cells can recognize and attack tumor cells with different antigen profiles. We experimentally validate circRNA circularization, antigen expression, dendritic cell maturation, and T-cell-mediated tumor cell killing. Input from clinicians and other stakeholders guides our iterative design. CirCure offers a scalable, "plug-and-play" framework for RNA vaccines against cancers in which tumor diversity limits single-target treatments. Wiki | Official Page Sat, 29 Aug 2026 17:16:44 GMT https://validator.w3.org/feed/docs/rss2.html PeerTube - https://video.igem.org https://video.igem.org/client/assets/images/icons/icon-1500x1500.png https://video.igem.org/videos/watch/a30c979a-6ad9-445a-8eb4-642071920339 All rights reserved, unless otherwise specified in the terms specified at https://video.igem.org/about and potential licenses granted by each content's rightholder.