https://video.igem.org/videos/watch/a76c1594-473f-4be5-bebb-429c67bbd369 Title: LEDA: Living Engineered Diagnostic Assay Description: A dosimeter records the dose that reached the badge. It cannot say what that dose did to the person wearing it. After a high exposure there is a latent window of up to three weeks in which the patient looks well while the damage continues, and the laboratory methods that answer the question need a specialist lab and hours of analysis. LEDA is a point-of-care assay for DNA damage. Engineered E. coli read two biomarkers from a urine sample. 8-oxodG reports that the cell has found oxidative damage and is processing it. Cell-free DNA reports cells that have already died. The first circuit imports the nucleoside, strips its sugar and opens a riboswitch onto a transcript pool the cell is already holding. The second reads the DNA at the membrane: a cfDNA fragment has two free ends, one receptor clamps each, and the fragment the test is looking for is also the part that completes the sensor. A reader we built, a light-tight box with a phone camera, quantifies both signals. Outside the lab the project was redrawn as much as it was built. The Association of the Blind of Northwestern Greece, the Tunnel of Silence, the Hellenic Association for the Protection and Rehabilitation of Disabled People, an elderly day centre and groups of schoolchildren changed the casing, the colours and the way the result is written, and they are the reason there is a fairy tale in sixteen languages and in Braille, three games, and project material in sign language. A professor of moral philosophy replaced our consent button with a document in everyday language. Three medical physicists at the University Hospital of Ioannina narrowed the use case from routine staff monitoring to the day a source leaks or equipment fails. The business model followed the same evidence. Hospitals and institutions buy LEDA and hand it to the people who take the test: a reusable reader at about forty euros, cartridges at about twenty, and an annual fee for the dashboard, calibration and support. The pilot is three to five Greek departments at five to fifteen thousand tests a year, break-even sits in mid-2030, and the cost that governs that schedule is IVDR conformity assessment rather than manufacturing. Wiki | Official Page Thu, 29 Oct 2026 22:23:07 GMT https://validator.w3.org/feed/docs/rss2.html PeerTube - https://video.igem.org https://video.igem.org/client/assets/images/icons/icon-1500x1500.png https://video.igem.org/videos/watch/a76c1594-473f-4be5-bebb-429c67bbd369 All rights reserved, unless otherwise specified in the terms specified at https://video.igem.org/about and potential licenses granted by each content's rightholder.