https://video.igem.org/videos/watch/ab6e5cff-6ff0-4972-88bf-3889a18048c3 This video presents a light-off control simulation of engineered Escherichia coli migration across a fluid–mucus interface using our agent-based model (ABM). The animated x–y occupancy heatmap visualizes the cumulative spatial distribution of bacterial trajectories over time, with brighter colors indicating higher cumulative occupancy. The dashed vertical lines delineate three modeled regions: the surrounding fluid, the superficial mucus layer, and the deeper mucus layer. Under this control condition, no blue-light input is applied to regulate bacterial motility. The simulation provides a baseline for comparing bacterial movement patterns, spatial distributions, and mucus-penetration outcomes under different light-control conditions. This video presents computational simulation results rather than experimental observations. https://2026.igem.wiki/ecust Mon, 19 Oct 2026 02:50:23 GMT https://validator.w3.org/feed/docs/rss2.html PeerTube - https://video.igem.org https://video.igem.org/client/assets/images/icons/icon-1500x1500.png https://video.igem.org/videos/watch/ab6e5cff-6ff0-4972-88bf-3889a18048c3 All rights reserved, unless otherwise specified in the terms specified at https://video.igem.org/about and potential licenses granted by each content's rightholder.