https://video.igem.org/videos/watch/bdb93276-b10b-47b4-a70b-03f420918e4d Title: RATAtouille, Multi-Layer Genetic Circuits for Dynamic Metabolic Control Description: Our team develops logic gate-based, multi-layer genetic circuits to dynamically control microbial cell factories. Conventional metabolic engineering maximizes gene expression, which impairs growth, accumulates toxic intermediates, and worsens the trade-off between growth and production. Instead, we build circuits that read the cellular state, including growth phase, population density, and metabolic condition, and select the appropriate output in response. By combining standardized input modules into hierarchical circuits, we tune the timing and strength of expression to match the cellular state. To demonstrate this, we showcase pigment production across a palette of carotenoids and violacein derivatives. The name RATAtouille captures this design: a Reprogrammable Autoregulatory Transcriptional Architecture, through which a cell senses and orchestrates its own response. It also evokes ratatouille, where varied ingredients keep their character while forming one harmonious plate. Likewise, our circuits read a cell's metabolic states and shape its flux so that growth and production move in harmony. Wiki | Official Page Tue, 01 Sep 2026 16:54:09 GMT https://validator.w3.org/feed/docs/rss2.html PeerTube - https://video.igem.org https://video.igem.org/client/assets/images/icons/icon-1500x1500.png https://video.igem.org/videos/watch/bdb93276-b10b-47b4-a70b-03f420918e4d All rights reserved, unless otherwise specified in the terms specified at https://video.igem.org/about and potential licenses granted by each content's rightholder.