https://video.igem.org/videos/watch/c0eb4e11-8560-4f90-9914-ed49388885b7 Summary. This video shows a 1-microsecond coarse-grained molecular dynamics simulation of the dCas9–sgRNA–ctDNA recognition complex, the molecular core of NEUMA’s biosensor for the early detection of EGFR-mutant lung cancer. Catalytically dead Cas9 (blue) holds the single-guide RNA (orange) and a circulating-tumour-DNA fragment carrying the EGFR exon-19 deletion (green). The 20-base-pair RNA:DNA heteroduplex that encodes the target recognition stays stable throughout the trajectory, while the free arms of the ctDNA progressively fold and adsorb onto the protein surface — showing how the complex engages and holds its target in solution over the microsecond. Credits. Dry-lab modelling and animation: NEUMA iGEM team. Structure prediction: AlphaFold 3. Molecular dynamics: GROMACS 2026.1 with the Martini 2 coarse-grained force field. Visualization and rendering: VMD. Video editing: ffmpeg. Team wiki:https://2026.igem.wiki/puebla/model Tue, 20 Oct 2026 02:54:58 GMT https://validator.w3.org/feed/docs/rss2.html PeerTube - https://video.igem.org https://video.igem.org/client/assets/images/icons/icon-1500x1500.png https://video.igem.org/videos/watch/c0eb4e11-8560-4f90-9914-ed49388885b7 All rights reserved, unless otherwise specified in the terms specified at https://video.igem.org/about and potential licenses granted by each content's rightholder.