https://video.igem.org/videos/watch/d4d2266a-8ff9-40b0-b297-15e87b9e2da4 Title: RHAea Description: Olive oil mill wastewater is a major environmental challenge in olive oil producing regions such as Crete. Its extremely high organic load, coupled with its high concentration of phenolic compounds, can severely affect aquatic ecosystems, soil and vegetation when poorly managed. RHAea aims to turn this waste stream into a valuable resource through a two-stage approach. First, we recover hydroxytyrosol using a chimeric protein composed of a cellulose-binding domain and the HyT12 mutant, connected by a flexible linker. The cellulose-binding domain of the protein anchors the protein to a cellulose-based material, creating a filter-like structure, while HyT12 specifically binds hydroxytyrosol. After phenolic recovery, the wastewater still contains a high organic load. Therefore, we use it as a substrate for rhamnolipid production with engineered Pseudomonas putida. Rhamnolipids are biodegradable biosurfactants with applications in detergents, cosmetics, agriculture and bioremediation. Official Page Tue, 01 Sep 2026 16:54:07 GMT https://validator.w3.org/feed/docs/rss2.html PeerTube - https://video.igem.org https://video.igem.org/client/assets/images/icons/icon-1500x1500.png https://video.igem.org/videos/watch/d4d2266a-8ff9-40b0-b297-15e87b9e2da4 All rights reserved, unless otherwise specified in the terms specified at https://video.igem.org/about and potential licenses granted by each content's rightholder.