https://video.igem.org/videos/watch/d58a7311-5312-4eb2-ac86-b0b51f682d95 Title: CLASP: Conditional Localized Assembly of Synthetic Proteins Description: Within living cells, functions emerge from the conditional spatial assembly of molecular components. Modern synthetic biology allows precise editing of gene expression, yet controlling post-translational spatial localization remains a critical bottleneck. Current conditional localization methods remain fundamentally limited, relying on exogenous chemicals or light, which suffers leakage and offers poorly controlled spatial geometry. To address this matter, we built CLASP– Conditional Localized Assembly of Synthetic Proteins, a modular protein scaffold framework that enables nanometer precision control of spatial proximity in response to target customisable signals. CLASP contains a rigid Consensus Tetratricopeptide Repeat (CTPR) as the system’s backbone, customizable sensor modules, molecular payloads, split-luciferase complementation for real-time quantification, and optional isopeptide covalent locking of the assembled state. Together, these features enable a programmable molecular assembly system: recruit, report, retain, and react. ORIGAMI, a mechanism-aware designed application, is also developed to support CLASP. By combining physics-based modeling and formulas with active-learning inference, ORIGAMI formalizes the relationship between nanoscale structure and spatial proximity, generating quantitative and testable models. ORIGAMI mechanistically informs protein-scaffold design, turning it into a closed-loop discovery. Informed by ORIGAMI, CLASP is demonstrated in two distinct applications: signal-responsive enzyme assembly under salinity stress and biomarker-induced proximity for diagnostic screening. These example use cases highlight that CLASP is an universal foundational system rather than any laboratory tool. CLASP provides a signal-responsive, geometrically programmable framework that transforms induced proximity into a deliberate engineering system. Intertwined closely in our designs and engineering cycles is Integrated Human Practices. With each decision, we consult professors in specialized niches like bioengineering, protein screening, and bioinformatics to provide us with invaluable insights to improve our project; like how Professor Millan reminded us to do more structural analyses, and how Professor Burgess-Brown suggested us to do variant screenings, and many more. With each interview, we aim to integrate their expert opinions in our engineering to build a more feasible, economically viable, and ethical CLASP. Education is a also an inseparable part of our project. Through a diverse array of active and passive schemes built deliberately on the “ACTION” framework designed by our team, we aim to cultivate engagement of synthetic biology in the cognitive and behavioural domains. With two-way communication and mutual learning, we improve our educational initiatives every time. It’s notable that we highlight inclusivity as the core value of our team, with the envisioned future of equal opportunity for all in mind, we target distinct social strata within our active blueprint, while ensuring that the passive schematics are comprehensive and extensive. Wiki | Official Page Thu, 29 Oct 2026 22:25:05 GMT https://validator.w3.org/feed/docs/rss2.html PeerTube - https://video.igem.org https://video.igem.org/client/assets/images/icons/icon-1500x1500.png https://video.igem.org/videos/watch/d58a7311-5312-4eb2-ac86-b0b51f682d95 All rights reserved, unless otherwise specified in the terms specified at https://video.igem.org/about and potential licenses granted by each content's rightholder.