https://video.igem.org/videos/watch/db3585b4-36fd-4555-a3dc-4041873d964a Title: Differphase: Construction of a Stably Differentiated Platform Based on Asymmetric Distribution via Phase Separation Description: Differphase is a synthetic biology platform that builds stable, programmable bacterial communities from a single founder cell. Using LLPS-driven asymmetric plasmid partitioning, it generates distinct functional cell types with tunable ratios. Four integrated modules maintain founder cells, drive differentiation, replenish the founder pool via blue-light-controlled dedifferentiation, and prevent plasmid loss through toxin-antitoxin systems. Experiments and mathematical modeling validated key components and population dynamics. Differphase addresses the trade-off between yield and stability in engineered microbes, offering promising applications in precision biomanufacturing, tumor therapy, and bioremediation. From one cell, we engineer a community; From differentiation, we build the future of biotechnology. Wiki | Official Page Thu, 29 Oct 2026 22:24:33 GMT https://validator.w3.org/feed/docs/rss2.html PeerTube - https://video.igem.org https://video.igem.org/client/assets/images/icons/icon-1500x1500.png https://video.igem.org/videos/watch/db3585b4-36fd-4555-a3dc-4041873d964a All rights reserved, unless otherwise specified in the terms specified at https://video.igem.org/about and potential licenses granted by each content's rightholder.