https://video.igem.org/videos/watch/ededf100-f698-4c8b-b499-8c436aa42f88 The gene says what to build; the host decides whether it can be built. This audiobook explains how synthetic biologists choose the living cell (or no cell at all) that turns DNA into a working product. Chapters: What Is a Host Organism? Why DNA needs a cell, why plasmids are so useful, and how E. coli changed insulin production The History of Host Organisms: from Koch’s pure cultures and E. coli K-12 to Cohen and Boyer, BioBricks, synthetic genomes and a recoded E. coli Criteria for Choosing a Host: risk groups, protein folding and modification, metabolic burden, codon usage and how practical the host is to work with A Tour of the Major Chassis: E. coli, Bacillus, Pseudomonas, Vibrio natriegens, yeasts, mammalian cells, plants, algae and cell-free systems The End: why there’s no single best chassis, only the one that fits your project A short, clear guide for students and iGEM teams picking a host for their first construct. Produced by iGEM VIT Vellore. https://2026.igem.wiki/vit-vellore Wed, 21 Oct 2026 02:55:09 GMT https://validator.w3.org/feed/docs/rss2.html PeerTube - https://video.igem.org https://video.igem.org/client/assets/images/icons/icon-1500x1500.png https://video.igem.org/videos/watch/ededf100-f698-4c8b-b499-8c436aa42f88 All rights reserved, unless otherwise specified in the terms specified at https://video.igem.org/about and potential licenses granted by each content's rightholder.