https://video.igem.org/videos/watch/f47cd9dc-c371-40fc-8f38-9ea3e9da833d This video presents an agent-based model (ABM) simulation of engineered Escherichia coli migration across a fluid–mucus interface under blue-light illumination. The animated x–y occupancy heatmap visualizes the cumulative spatial distribution of bacterial trajectories over time, with brighter colors indicating higher accumulated occupancy. The dashed vertical lines delineate three modeled regions: the surrounding fluid, the superficial mucus layer, and the deeper mucus layer. In the model, blue-light-dependent modulation of CheY/CheZ-associated motility signaling alters bacterial run-and-tumble behavior. The simulation is used to investigate how light-regulated motility influences bacterial migration patterns and penetration into the mucus layers. This video presents computational simulation results rather than experimental observations. https://2026.igem.wiki/ecust Mon, 19 Oct 2026 02:53:11 GMT https://validator.w3.org/feed/docs/rss2.html PeerTube - https://video.igem.org https://video.igem.org/client/assets/images/icons/icon-1500x1500.png https://video.igem.org/videos/watch/f47cd9dc-c371-40fc-8f38-9ea3e9da833d All rights reserved, unless otherwise specified in the terms specified at https://video.igem.org/about and potential licenses granted by each content's rightholder.