Main smetzler channel https://video.igem.org/c/smetzler_channel/videos Wed, 21 Oct 2026 02:50:45 GMT https://validator.w3.org/feed/docs/rss2.html PeerTube - https://video.igem.org Main smetzler channel https://video.igem.org/client/assets/images/icons/icon-1500x1500.png https://video.igem.org/c/smetzler_channel/videos All rights reserved, unless otherwise specified in the terms specified at https://video.igem.org/about and potential licenses granted by each content's rightholder. https://video.igem.org/feeds/podcast/videos.xml?videoChannelId=4119 <![CDATA[Heidelberg: 12h HEK cell timelapse on the OpenFlexure, defocused passes filled by interpolation (2026)]]> https://video.igem.org/w/m5rUE2v2hAnHPzaSqHchYW https://video.igem.org/w/m5rUE2v2hAnHPzaSqHchYW Tue, 20 Oct 2026 11:16:35 GMT The same 12 h HEK timelapse as the corrected version, but the passes that lost focus are filled by frame interpolation between the sharp neighbours. It is a workaround for display, not a fix: the real fix was switching to darkfield, where cells give a proper focus peak. Part of the VOYAGE project by iGEM Heidelberg 2026.

https://2026.igem.wiki/heidelberg

]]> Main smetzler channel Science & Technology nonadult Heidelberg: 12h HEK cell timelapse on the OpenFlexure, defocused passes filled by interpolation (2026) The same 12 h HEK timelapse as the corrected version, but the passes that lost focus are filled by frame interpolation between the sharp neighbours. It is a workaround for display, not a fix: the real fix was switching to darkfield, where cells g... <![CDATA[Heidelberg: 12h HEK cell timelapse on the OpenFlexure, background and flat-field corrected (2026)]]> https://video.igem.org/w/3XREDLY7XsHKnmomqGRTQH https://video.igem.org/w/3XREDLY7XsHKnmomqGRTQH Tue, 20 Oct 2026 11:15:43 GMT HEK cells imaged in brightfield inside the incubator for 12 hours on our OpenFlexure microscope: a 3×3 tile mosaic, 79 passes. Background- and flat-field corrected only. The passes where autofocus failed are left in, which shows the problem we hit: unstained cells are phase objects and give standard autofocus no clear focus peak. Part of the VOYAGE project by iGEM Heidelberg 2026.

https://2026.igem.wiki/heidelberg

]]> Main smetzler channel Science & Technology nonadult Heidelberg: 12h HEK cell timelapse on the OpenFlexure, background and flat-field corrected (2026) HEK cells imaged in brightfield inside the incubator for 12 hours on our OpenFlexure microscope: a 3×3 tile mosaic, 79 passes. Background- and flat-field corrected only. The passes where autofocus failed are left in, which shows the problem we hi...